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Petrov, E.P.* ; Czogalla, A.* ; Kauert, D.J.* ; Seidel, R.* ; Schwille, P.*

Diffusion and freezing transition of rod-like DNA origami on freestanding lipid membranes.

Biophys. J. 106:701a (2014)

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During the last decade, DNA origami has become a powerful tool in research at the nanoscale. The relative ease of constructing functionalized DNA origami structures of a defined shape allows for their applications in membrane biophysics. Recently, we have constructed stiff rod-like DNA origami structures consisting of six DNA helixes, which were functionalized with hydrophobic membrane-binding anchors and fluorescently labeled at defined positions [1]. Selective fluorescent labeling allowed us to determine the translational and rotational diffusion coefficients of the DNA origami rods on lipid membranes by fluorescence correlation spectroscopy, which were found to be in a good agreement with the hydrodynamics-based theory of membrane diffusion.

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