PuSH - Publication Server of Helmholtz Zentrum München

Heinzmann, S.S. ; Schmitt-Kopplin, P.

Deep metabotyping of the murine gastrointestinal tract for the visualization of digestion and microbial metabolism.

J. Proteome Res. 14, 2267-2277 (2015)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Despite the gut's longitudinal specialization for digestion and microbiome organization, most studies focus on the analysis of its end product, feces. To determine the metabolic and physiological functions of different sections of the gut, we aimed to define a comprehensive list of characteristic metabolites for the physiological gut sections and to quantify the selected pathways. We investigated the metabolic composition of seven different gut sections from four C57Bl/6N mice with nontargeted metabolite profiling using high-resolution NMR spectroscopy, which returned a comprehensive metabolite overview with a single analytical measurement per sample. Here we deliver a list of characteristic metabolites, describe metabolite changes along the gut, and highlight the site specificity for selected metabolite pathways. We find that the largest metabolic changes happen in the cecum, where the microbiome produces microbial metabolites. Furthermore, we show the evolution of bile acids along the gut and describe their site-specific conversion. We establish a metabolic basis for future investigations of metabolic perturbations, which can be introduced by dietary challenges or gene knockouts and provide valuable information for tailored study design and targeted sample collection.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Nmr Spectroscopy ; Bile Acids ; Gastrointestinal Physiology ; Gut Microbiota ; Metabolite Profiling; Gut Microbiota; Bile-acids; Molecular Analysis; Human Stomach; Carbohydrate; Health; Identification; Consequences; Fermentation; Conversion
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Journal Journal of Proteome Research
Quellenangaben Volume: 14, Issue: 5, Pages: 2267-2277 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Publishing Place Washington
Reviewing status Peer reviewed
Institute(s) Research Unit BioGeoChemistry and Analytics (BGC)
German Center for Diabetes Reseach (DZD)