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Sezgin, E.* ; Gutmann, T. ; Buhl, T.* ; Dirkx, R. ; Grzybek, M. ; Coskun, Ü. ; Solimena, M. ; Simons, K.* ; Levental, I.* ; Schwille, P.*

Adaptive lipid packing and bioactivity in membrane domains.

PLoS ONE 10:e0123930 (2015)
Publ. Version/Full Text DOI PMC
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Lateral compositional and physicochemical heterogeneity is a ubiquitous feature of cellular membranes on various length scales, from molecular assemblies to micrometric domains. Segregated lipid domains of increased local order, referred to as rafts, are believed to be prominent features in eukaryotic plasma membranes; however, their exact nature (i.e. size, lifetime, composition, homogeneity) in live cells remains difficult to define. Here we present evidence that both synthetic and natural plasma membranes assume a wide range of lipid packing states with varying levels of molecular order. These states may be adapted and specifically tuned by cells during active cellular processes, as we show for stimulated insulin secretion. Most importantly, these states regulate both the partitioning of molecules between coexisting domains and the bioactivity of their constituent molecules, which we demonstrate for the ligand binding activity of the glycosphingolipid receptor GM1. These results confirm the complexity and flexibility of lipid-mediated membrane organization and reveal mechanisms by which this flexibility could be functionalized by cells.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cell-membranes; Generalized Polarization; Fluorescent-probe; Plasma-membranes; Raft Mixtures; Vesicles; Model; Laurdan; Phases; Microscopy
Language english
Publication Year 2015
HGF-reported in Year 2015
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 10, Issue: 4, Pages: , Article Number: e0123930 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)
POF-Topic(s) 90000 - German Center for Diabetes Research
Research field(s) Helmholtz Diabetes Center
PSP Element(s) G-502600-002
G-502600-001
PubMed ID 25905447
DOI 10.1371/journal.pone.0123930
WOS ID WOS:000353332000049
Erfassungsdatum 2015-04-25
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