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Serial sectioning and multispectral imaging system for versatile biomedical applications.
In: Proceedings (IEEE International Symposium on Biomedical Imaging, ISBI, 29. April - 02.May 2014, Beijing, China). IEEE: Piscataway, NJ, 2014. 890-893
Serial sectioning combined with microscopy provides high resolution volumetric data to complement in-vivo imaging modalities and aid ex-vivo diagnostics. We describe the design of a fully-automated cryomicrotome combined with a multispectral reflection and fluorescence imaging system that enables high-throughput analyses of biological specimens with a large field of view and cellular resolution while keeping the manufacturing and running costs low. We show the performance of the system for representative applications in high-resolution volumetric imaging of reporter animals and multispectral tissue analysis. Furthermore, we demonstrate the versatility of the economical imaging system in applications such as in vivo epifluorescence imaging, histology slide scanning, cell counting and gel electrophoresis documentation.
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Publication type
Article: Conference contribution
Keywords
3d Visualization ; Cryo-microtome Serial Tissue Sectioning ; Cryoslicer Imaging ; Epifluorescence Imaging ; Multispectral Imaging
ISBN
9781467319591
Conference Title
IEEE International Symposium on Biomedical Imaging, ISBI
Conference Date
29. April - 02.May 2014
Conference Location
Beijing, China
Proceedings Title
Proceedings
Quellenangaben
Pages: 890-893
Publisher
Piscataway, NJ
Publishing Place
IEEE
Non-patent literature
Publications
Institute(s)
Institute of Biological and Medical Imaging (IBMI)