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Open Access Green as soon as Postprint is submitted to ZB.
The CR4 region of EBNA2 convers viability of Epstein-Barr virus-transformed B cells by CBF1-independent signalling.
J. Gen. Virol. 87, 3169-3176 (2006)
The Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) gene product is the key regulator of the latent genes of EBV and essential for EBV-mediated transformation of human primary B cells. Viral mutants were constructed carrying a deletion of the EBNA2 conserved region 4 (CR4). Primary resting B cells infected with the DeltaCR4-EBNA2 mutant virus were dramatically impaired for B cell transformation. Lymphoblastoid cell lines (LCLs) established with this mutant EBV revealed a prolonged population doubling time when cells were cultivated at low cell densities, which are not critical for wild-type-infected cells. Low-level spontaneous cell death occurred when the cells were cultivated at suboptimal cell densities. The phenotype of B cells and LCLs infected with the DeltaCR4-EBNA2 mutant virus indicated that the CR4 region of EBNA2 specifically contributes to the viability of the cells rather than affecting cell division rates.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0022-1317
e-ISSN
1465-2099
Journal
Journal of General Virology
Quellenangaben
Volume: 87,
Issue: 11,
Pages: 3169-3176
Publisher
Society for General Microbiology
Reviewing status
Peer reviewed
Institute(s)
Research Unit Gene Vector (AGV)