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Retrieving small features in reflection-mode Raster-Scan Optoacoustic Mesoscopy (RSOM) using multi-frequency reconstruction.
Proc. SPIE 9323:932326 (2015)
Publ. Version/Full Text
DOI
We developed a reflection-mode, raster-scan optoacoustic mesoscopy system, based on a custom-made ultrasonic detector, with an ultra wide bandwidth of 20-180 MHz. To optimally use this bandwidth, we implemented multi-frequency reconstruction. System characterization reveals a 4 μm axial, and 18 μm transverse resolution, at penetration depths reaching 5 mm. After characterization, the system was applied to image a zebrafish ex vivo, and an excised mouse ear. In the zebrafish, the lateral line, intestines, eyes, and melanocytes are seen, while in the mouse ear, multi-frequency reconstruction recovered the small vessels, otherwise not seen on the image.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Beam-forming ; Epi-illumination ; High Frequency ; Mesoscopy ; Microscopy ; Optoacoustics
ISSN (print) / ISBN
0277-786X
e-ISSN
1996-756X
Conference Title
Photons Plus Ultrasound Imaging and Sensing 2015
Conference Date
8.-10. February 2015
Conference Location
San Francisco, USA
Journal
Proceedings of SPIE
Quellenangaben
Volume: 9323,
Article Number: 932326
Publisher
SPIE
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)