PuSH - Publication Server of Helmholtz Zentrum München: Identification of two arginine kinase forms of endoparasitoid <em>Leptomastix dactylopii</em> venom by bottom up-sequence tag approach.

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Labella, C.* ; Kanawati, B. ; Vogel, H.* ; Schmitt-Kopplin, P. ; Laurino, S.* ; Bianco, G.* ; Falabella, P.*

Identification of two arginine kinase forms of endoparasitoid Leptomastix dactylopii venom by bottom up-sequence tag approach.

J. Mass Spectrom. 50, 756-765 (2015)

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Open Access Green as soon as Postprint is submitted to ZB.

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Leptomastix dactylopii (Howard) is an endoparasitoid wasp, natural enemy of mealybug Planococcus citri (Risso). Despite the acquired knowledge regarding this host-parasitoid interaction, only little information is available on the factors of parasitoid origin able to modulate the mealybug physiology. The major alteration observed in P. citri is a strong reduction in fecundity, which is evident soon after parasitization by L. dactylopii or venom injection in unparasitized hosts indicating that this proteinaceus secretion injected at the oviposition plays a key-role in host regulation. Protein identification of L. dactilopii venom has been limited by the lack of literature sources and public protein databases. Here, we identified two venom proteins by an integrated trascriptomic and proteomic approach. A custom-made transcriptomic database from the L. dactylopii venom glands was created by applying the high-throughput RNA sequencing approach. Two-dimensional gel electrophoresis (2DE) trypsinized protein spots were analyzed by high-resolution mass spectrometry (FTICRMS-12 T). The most abundant peptide ions were fragmented by collision induced dissociation and the obtained sequence tags were subjected to custom-made protein database searching. Two putative arginine kinases (full-length and truncated form) were identified. This is the first case in which both, truncated and full length arginine kinases, are identified in an endoparasitoid non-paralyzing venom.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords parasitoid;venom;high resolution mass spectrometry;bottom up sequence-tag;arginine kinase

ISSN (print) / ISBN 1076-5174

e-ISSN 1096-9888

Journal Journal of Mass Spectrometry

Quellenangaben Volume: 50, Issue: 5, Pages: 756-765

Publisher Wiley

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Analytical BioGeoChemistry (BGC)