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Keller, I.E. ; Vosyka, O. ; Takenaka, S. ; Kloß, A.* ; Dahlmann, B.* ; Willems, L.I.* ; Verdoes, M.* ; Overkleeft, H.S.* ; Marcos, E.* ; Adnot, S.* ; Hauck, S.M. ; Ruppert, C.* ; Günther, A.* ; Herold, S.* ; Ohno, S. ; Adler, H. ; Eickelberg, O. ; Meiners, S.

Regulation of immunoproteasome function in the lung.

Sci. Rep. 5:10230 (2015)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Impaired immune function contributes to the development of chronic obstructive pulmonary disease (COPD). Disease progression is further exacerbated by pathogen infections due to impaired immune responses. Elimination of infected cells is achieved by cytotoxic CD8(+)  T cells that are activated by MHC I-mediated presentation of pathogen-derived antigenic peptides. The immunoproteasome, a specialized form of the proteasome, improves generation of antigenic peptides for MHC I presentation thereby facilitating anti-viral immune responses. However, immunoproteasome function in the lung has not been investigated in detail yet. In this study, we comprehensively characterized the function of immunoproteasomes in the human and murine lung. Parenchymal cells of the lung express low constitutive levels of immunoproteasomes, while they are highly and specifically expressed in alveolar macrophages. Immunoproteasome expression is not altered in whole lung tissue of COPD patients. Novel activity-based probes and native gel analysis revealed that immunoproteasome activities are specifically and rapidly induced by IFNγ treatment in respiratory cells in vitro and by virus infection of the lung in mice. Our results suggest that the lung is potentially capable of mounting an immunoproteasome-mediated efficient adaptive immune response to intracellular infections.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Obstructive Pulmonary-disease; Endoplasmic-reticulum Stress; Cigarette-smoke; Epithelial-cells; Rhinovirus Infection; Antigen Presentation; Proteasome Activity; Dendritic Cells; Mice; Alveolar
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 5, Issue: , Pages: , Article Number: 10230 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Lung Health and Immunity (LHI)
Research Unit Gene Vector (AGV)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Lung Biology (LHI)