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Sadic, D.* ; Schmidt, K.* ; Groh, S.* ; Kondofersky, I. ; Ellwart, J.W. ; Fuchs, C. ; Theis, F.J. ; Schotta, G.*

Atrx promotes heterochromatin formation at retrotransposons.

EMBO Rep. 16, 836-850 (2015)
Publ. Version/Full Text DOI PMC
Open Access Gold
More than 50% of mammalian genomes consist of retrotransposon sequences. Silencing of retrotransposons by heterochromatin is essential to ensure genomic stability and transcriptional integrity. Here, we identified a short sequence element in intracisternal A particle (IAP) retrotransposons that is sufficient to trigger heterochromatin formation. We used this sequence in a genome-wide shRNA screen and identified the chromatin remodeler Atrx as a novel regulator of IAP silencing. Atrx binds to IAP elements and is necessary for efficient heterochromatin formation. In addition, Atrx facilitates a robust and largely inaccessible heterochromatin structure as Atrx knockout cells display increased chromatin accessibility at retrotransposons and non-repetitive heterochromatic loci. In summary, we demonstrate a direct role of Atrx in the establishment and robust maintenance of heterochromatin.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Atrx ; Daxx ; Iap Retrotransposons ; Heterochromatin ; Histone H3.3; Embryonic Stem-cells; Dna Methylation; Endogenous Retroviruses; Mental-retardation; Histone Chaperone; Repressor Domain; Protein Daxx; X Syndrome; Tif1 Beta; Chromatin
Language english
Publication Year 2015
HGF-reported in Year 2015
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Journal EMBO Reports
Quellenangaben Volume: 16, Issue: 7, Pages: 836-850 Article Number: , Supplement: ,
Publisher EMBO Press
Reviewing status Peer reviewed
Institute(s) Institute of Computational Biology (ICB)
Institute of Molecular Immunology (IMI)
POF-Topic(s) 30205 - Bioengineering and Digital Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Enabling and Novel Technologies
Immune Response and Infection
PSP Element(s) G-503800-001
G-501793-001
PubMed ID 26012739
DOI 10.15252/embr.201439937
Scopus ID 84934789462
Scopus ID 84929889600
WOS ID WOS:000357325800010
Erfassungsdatum 2015-05-29
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