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Headspace measurements of irradiated in vitro cultured cells using PTR-MS.
Radiat. Environ. Biophys. 50, 209-217 (2011)
A pilot study was performed to evaluate a new concept for a radiation biodosimetry method. Proton transfer reaction-mass spectrometry (PTR-MS) was used to find out whether radiation induces changes in the composition of volatile organic compounds (VOCs) in the headspace of in vitro cultured cells. Two different cell lines, retinal pigment epithelium cells hTERT-RPE1 and lung epithelium cells A-549, were irradiated with gamma radiation at doses of 4 Gy and 8 Gy. For measuring the cell-specific effects, the VOC concentrations in the headspace of flasks containing cells plus medium, as well as of flasks containing pure medium were analyzed for changes before and after irradiation. No significant radiation-induced alterations in VOC concentrations in the headspace could be observed after irradiation.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
volatile organic compounds (VOCs); PTR-MS; cell culture; radiation biodosimetry
Language
Publication Year
2011
Prepublished in Year
2010
HGF-reported in Year
2010
ISSN (print) / ISBN
0301-634X
e-ISSN
1432-2099
Quellenangaben
Volume: 50,
Issue: 1,
Pages: 209-217
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Research Unit Medical Radiation Physics and Diagnostics (AMSD)
Institute of Radiation Biology (ISB)
Institute of Radiation Biology (ISB)
POF-Topic(s)
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30202 - Environmental Health
30202 - Environmental Health
Research field(s)
Radiation Sciences
PSP Element(s)
G-503600-001
G-503600-002
G-500200-001
G-500200-002
G-503600-002
G-500200-001
G-500200-002
PubMed ID
20821023
Scopus ID
79952439754
Erfassungsdatum
2010-12-10