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Headspace measurements of irradiated in vitro cultured cells using PTR-MS.

Radiat. Environ. Biophys. 50, 209-217 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
A pilot study was performed to evaluate a new concept for a radiation biodosimetry method. Proton transfer reaction-mass spectrometry (PTR-MS) was used to find out whether radiation induces changes in the composition of volatile organic compounds (VOCs) in the headspace of in vitro cultured cells. Two different cell lines, retinal pigment epithelium cells hTERT-RPE1 and lung epithelium cells A-549, were irradiated with gamma radiation at doses of 4 Gy and 8 Gy. For measuring the cell-specific effects, the VOC concentrations in the headspace of flasks containing cells plus medium, as well as of flasks containing pure medium were analyzed for changes before and after irradiation. No significant radiation-induced alterations in VOC concentrations in the headspace could be observed after irradiation.
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Publication type Article: Journal article
Document type Scientific Article
Keywords volatile organic compounds (VOCs); PTR-MS; cell culture; radiation biodosimetry
Language
Publication Year 2011
Prepublished in Year 2010
HGF-reported in Year 2010
ISSN (print) / ISBN 0301-634X
e-ISSN 1432-2099
Quellenangaben Volume: 50, Issue: 1, Pages: 209-217 Article Number: , Supplement: ,
Publisher Springer
Reviewing status Peer reviewed
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30202 - Environmental Health
Research field(s) Radiation Sciences
PSP Element(s) G-503600-001
G-503600-002
G-500200-001
G-500200-002
PubMed ID 20821023
Scopus ID 79952439754
Erfassungsdatum 2010-12-10