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Tohge, T.* ; Zhang, Y.* ; Peterek, S.* ; Matros, A.* ; Rallapalli, G.* ; Tandrón, Y.A.* ; Butelli, E.* ; Kallam, K.* ; Hertkorn, N. ; Mock, H.P.* ; Martin, C.* ; Fernie, A.R.*

Ectopic expression of snapdragon transcription factors facilitates the identification of genes encoding enzymes of anthocyanin decoration in tomato.

Plant J. 83, 686-704 (2015)
Publ. Version/Full Text Postprint DOI PMC
Open Access Green
Given the potential health benefits of polyphenolic compounds in the diet, there is a growing interest in the generation of food crops enriched with health-protective flavonoids. We undertook a series of metabolite analyses of tomatoes ectopically expressing the Delila and Rosea1 transcription factor genes from snapdragon, paying particular attention to changes in phenylpropanoids compared to controls. These analyses revealed multiple changes including depletion of rutin and naringenin-chalcone, enhanced levels of anthocyanins and phenylacylated flavonol derivatives. We isolated and characterised the chemical structures of the two most abundant anthocyanins which were shown by nuclear magnetic resonance spectroscopy to be delphinidin-3-(4'''-O-trans-p-coumaroyl)-rutinoside-5-O-glucoside and petunidin-3-(4'''-O-trans-p-coumaroyl)-rutinoside-5-O-glucoside. By performing RNA sequencing on both the purple fruit and wild type fruit we collected important information concerning the relative expression of both structural and transcription factor genes. Integrative analysis of the transcript and metabolite datasets provided compelling evidence of the nature of all genes involved in anthocyanin biosynthesis including those encoding species-specific anthocyanin decoration enzymes. One gene, SlFdAT1 (Solyc12g088170), predicted to encode a flavonoid-3-O-rutinoside-4'''-phenylacyltransferase was characterized by assays of recombinant protein and overexpression assays in tobacco. The combined data are discussed in the context of both our current understanding of phenylpropanoid metabolism in Solanaceous species, and evolution of flavonoid decorating enzymes and their transcriptional networks in different plant species.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Gc/ms Profiling ; Lc/ms Profiling ; Rnaseq ; Solanaceous Species ; Acyltransferase ; Anthocyanin Metabolism ; Glycosyltransferase ; Tomato; Arabidopsis-thaliana; Fruit-development; Convergent Evolution; Integrated Analysis; Metabolic Shifts; Free-radicals; Wild Tomato; Biosynthesis; Plants; Flavonoids
Language english
Publication Year 2015
HGF-reported in Year 2015
ISSN (print) / ISBN 0960-7412
e-ISSN 1365-313X
Journal Plant Journal, The
Quellenangaben Volume: 83, Issue: 4, Pages: 686-704 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Reviewing status Peer reviewed
Institute(s) Research Unit BioGeoChemistry and Analytics (BGC)
POF-Topic(s) 30202 - Environmental Health
Research field(s) Environmental Sciences
PSP Element(s) G-504800-001
PubMed ID 26108615
DOI 10.1111/tpj.12920
WOS ID WOS:000359356300010
Scopus ID 84938767205
Erfassungsdatum 2015-07-01
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