A proof of concept for the simultaneous multi-parameter determination of three inflammation and sepsis parameters-TNFα, PCT and CRP-using a compact optical immunosensor is demonstrated. Harmonized assay conditions revealed standard curves with test midpoints (IC(50)) of 380 µg L(-1) for TNFα, 2300 µg L(-1) for PCT, and 2645 µg L(-1) for CRP.