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Open Access Green as soon as Postprint is submitted to ZB.
dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.
Mol. Cell. Biol. 28, 2745-2757 (2008)
ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase II to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0270-7306
e-ISSN
1098-5549
Journal
Molecular and Cellular Biology
Quellenangaben
Volume: 28,
Issue: 8,
Pages: 2745-2757
Publisher
American Society for Microbiology (ASM)
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Molecular Immunology (IMI)