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Open Access Green as soon as Postprint is submitted to ZB.
The potential of retroviral vectors to cotransfer human endogenous retroviruses (HERVs) from human packaging cell lines.
Gene 390, 175-179 (2007)
Using a versatile and highly sensitive retroviral microarray, we have investigated particle preparations from three different human packaging cell lines harboring retroviral vector systems based on human immunodeficiency virus (HIV) and murine leukemia virus (MLV). 293Rev/Gag/Poli cells inducibly express high titers of HIV-derived particles for packaging of HIV vectors. The Phoenix-GP and the Anjou 65 cell lines constitutively express MLV vector particles. We compared the transcription profiles of human endogenous retroviruses (HERVs) in all cell lines with the HERV sequences present in the particles. In addition, the influence of the transfected vector plasmid on the copackaging of HERVs was investigated. All particle preparations showed a defined pattern of endogenous retroviral sequences that differed from the cellular HERV expression pattern. HERV transcripts were observed in the particle preparations independent of whether a vector construct was coexpressed or not.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Human endogenous retroviruses; HERV; Retroviral vector; Packaging cell line; Copackaging; DNA chip; Microarray; gene therapy
ISSN (print) / ISBN
0378-1119
e-ISSN
1879-0038
Journal
Gene
Quellenangaben
Volume: 390,
Issue: 1-2,
Pages: 175-179
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)