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as soon as is submitted to ZB.
In vivo retrograde labeling of neurons in the zebrafish embryo or larva with rhodamine dextran.
Cold Spring Harb. Protoc. 7, pdb.prot4832 (2007)
Application of a fluorescent dye to a target area of axons or dendrites allows the identification of individual neurons projecting to the area and reconstruction of the tract of the neurite by retrograde labeling. For the red fluorescent dye tetramethylrhodamine dextran, the labeling occurs by active retrograde transport inside the cell. This protocol describes a method for applying tetramethylrhodamine dextran to living zebrafish embryos and larvae (2-7 dpf [days post-fertilization]) via microinjection. It may be adapted for use with green or yellow fluorescent dextran conjugates.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1940-3402
e-ISSN
1559-6095
Journal
Cold Spring Harbor Protocols
Quellenangaben
Volume: 7,
Pages: pdb.prot4832
Publisher
Cold Spring Harbor Laboratory Press
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)