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Razansky, D. ; Ntziachristos, V.

Combining light and sound – multispectral optoacoustic tomography for high resolution molecular imaging.

Vortrag: European Society for Molecular Imaging (ESMI) 2009 Hot Topic Meeting: Multi-modality Imaging, 28th January 2009, Les Houches, France. (2009)
We have developed a multi-spectral optoacoustic tomography (MSOT) method capable of high resolution visualization of molecular bio-markers, including fluorescent proteins and probes, deep within living optically diffusive organisms. Methods: The method is based on ultrasonic detection of pressure waves generated by the absorption of pulsed light in elastic media. As such, it offers three-dimensional tomographic ability of optical contrast in conjunction with high spatial resolution resulting from diffraction-limited ultrasonic detection. Additionally, we employ multispectral illumination along with accurate normalization for photon propagation in tissues in order to provide quantified visualization of molecular probe distribution. Results: Using MSOT, we were able to achieve high resolution whole-body visualization of flurescent probe distribution in mice as well as tissue-specific expression of eGFP and mCherrry fluorescent proteins in Drosophila melanogaster pupa and the adult zebrafish. So far, those widely used model organisms were not accessible at their particular developmental stages by any of the existing optical microscopy techniques due to an extensive light scattering. For our method, we further demonstrate single cell sensitivity and penetration depths of centimeter and beyond in tissue-mimicking phantom experiments. Conclusion: This technology fills a significant area in biological imaging that goes way beyond the penetration limit of modern microscopy and can become the method of choice in studying signaling pathways and gene expression, morphogenesis, decease progression and many other targeted mechanisms through whole bodies of opaque living organisms and animals with high spatial resolution and sensitivity.
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Publication type Other: Lecture
Corresponding Author
Conference Title European Society for Molecular Imaging (ESMI) 2009 Hot Topic Meeting: Multi-modality Imaging
Conference Date 28th January 2009
Conference Location Les Houches, France
Non-patent literature Publications
Institute(s) Institute of Biological and Medical Imaging (IBMI)