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Haslbeck, V.* ; Drazic, A.* ; Eckl, J.M.* ; Alte, F.* ; Helmuth, M.* ; Popowicz, G.M. ; Rattei, T. ; Braun, F.* ; Weiwad, M.* ; Fischer, G.* ; Gemmecker, G.* ; Sattler, M. ; Striggow, F.* ; Groll, M.* ; Richter, K.*

Selective activators of protein phosphatase 5 target the auto-inhibitory mechanism.

Biosci. Rep. 35:e00210 (2015)
Publ. Version/Full Text Supplement DOI PMC
Open Access Gold
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Protein phosphatase 5 (PP5) is an evolutionary conserved serine/threonine phosphatase. Its dephosphorylation activity modulates a diverse set of cellular factors including protein kinases and the microtubule-associated tau protein involved in neurodegenerative disorders. It is auto-regulated by its heat-shock protein (Hsp90)-interacting tetratricopeptide repeat (TPR) domain and its C-terminal α-helix. In the present study, we report the identification of five specific PP5 activators [PP5 small-molecule activators (P5SAs)] that enhance the phosphatase activity up to 8-fold. The compounds are allosteric modulators accelerating efficiently the turnover rate of PP5, but do barely affect substrate binding or the interaction between PP5 and the chaperone Hsp90. Enzymatic studies imply that the compounds bind to the phosphatase domain of PP5. For the most promising compound crystallographic comparisons of the apo PP5 and the PP5-P5SA-2 complex indicate a relaxation of the auto-inhibited state of PP5. Residual electron density and mutation analyses in PP5 suggest activator binding to a pocket in the phosphatase/TPR domain interface, which may exert regulatory functions. These compounds thus may expose regulatory mechanisms in the PP5 enzyme and serve to develop optimized activators based on these scaffolds.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Modulation Of Phosphatase Activity ; Protein Phosphatase 5 ; Small-molecular Activators; Tetratricopeptide Repeat; Alzheimers-disease; Caenorhabditis-elegans; Tpr Proteins; Tau; Hsp90; Protein-phosphatase-5; Dephosphorylation; Phosphorylation; Complexes
Language english
Publication Year 2015
HGF-reported in Year 2015
ISSN (print) / ISBN 0144-8463
e-ISSN 1573-4935
Journal Bioscience Reports
Quellenangaben Volume: 35, Issue: 3, Pages: , Article Number: e00210 Supplement: ,
Publisher Portland Press
Publishing Place Colchester
Reviewing status Peer reviewed
Institute(s) Institute of Structural Biology (STB)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503000-001
G-503000-003
PubMed ID 26182372
DOI 10.1042/BSR20150042
WOS ID WOS:000357105800019
Scopus ID 84934981978
Erfassungsdatum 2015-07-17
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