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Scavizzi, F.* ; Ryder, E.* ; Newman, S.* ; Raspa, M.* ; Gleeson, D.* ; Wardle-Jones, H.* ; Montoliu, L.* ; Fernandez, A.* ; Dessain, M.-L.* ; Larrigaldie, V.* ; Khorshidi, Z.* ; Voulteenaho, R.* ; Soininen, R.* ; André, P.* ; Jacquot, S.* ; Hong, Y. ; Hrabě de Angelis, M. ; Ramirez-Solis, R.* ; Doe, B.*

Blastocyst genotyping for quality control of mouse mutant archives: An ethical and economical approach.

Transgenic Res. 24, 921-927 (2015)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
With the advent of modern developmental biology and molecular genetics, the scientific community has generated thousands of newly genetically altered strains of laboratory mice with the aim of elucidating gene function. To this end, a large group of Institutions which form the International Mouse Phenotyping Consortium is generating and phenotyping a knockout mouse strain for each of the ~20,000 protein-coding genes using the mutant ES cell resource produced by the International Knockout Mouse Consortium. These strains are made available to the research community via public repositories, mostly as cryopreserved sperm or embryos. To ensure the quality of this frozen resource there is a requirement that for each strain the frozen sperm/embryos are proven able to produce viable mutant progeny, before the live animal resource is removed from cages. Given the current requirement to generate live pups to demonstrate their mutant genotype, this quality control check necessitates the use and generation of many animals and requires considerable time, cage space, technical and economic resources. Here, we describe a simple and efficient method of genotyping pre-implantation stage blastocysts with significant ethical and economic advantages especially beneficial for current and future large-scale mouse mutagenesis projects.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords 3r’s ; Cryopreservation ; Mouse ; Network Of Repositories ; Pcr ; Quality Control (qc)
ISSN (print) / ISBN 0962-8819
e-ISSN 1573-9368
Journal Transgenic Research
Quellenangaben Volume: 24, Issue: 5, Pages: 921-927 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)