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Open Access Green as soon as Postprint is submitted to ZB.
An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence.
Plant Mol. Biol. 34, 417-426 (1997)
Stilbene synthase (STS) is an enzyme involved in the biosynthesis of stilbenes, which are synthesized in various plants in response to pathogen attack, UV irradiation or exposure to ozone. We describe analysis of an ozone inducible STS transcript and its corresponding promoter (Vst1), combined with the beta-glucuronidase (GUS) reporter gene. A single ozone pulse (0.1 microliter/l, 10 h) resulted in 11-fold GUS expression. Histochemical localization of GUS activity revealed small spots distributed over the whole leaf. Cross-sections of leaf tissue showed that the Vst1 promoter was induced in palisade and spongy parenchyma cells and to a lesser extent in epidermal cells. Deletions at the 5' end showed that a partial promoter sequence between position -430 and -280 constituted the ozone-responsive region, whereas for effective pathogen-inducibility sequences from -280 to -140 have been shown to be necessary.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0167-4412
e-ISSN
0167-4412
Journal
Plant Molecular Biology
Quellenangaben
Volume: 34,
Issue: 3,
Pages: 417-426
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)