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Open Access Green as soon as Postprint is submitted to ZB.
A simple method of DNA extraction from soil for detection of composite transgenic plants.
Plant Mol. Biol. Rep. 14, 143-148 (1996)
This new and simple method of DNA extraction from composite soil allows the isolation of plant DNA with high efficiency, quality and reproductivity. The method is based on a simple CaCl2-precipitation step and requires no additional purification steps to eliminate humic acids. The extracted DNA was obtained in sufficient purity and quantity to allow direct detection of transgenes by PCR. Furthermore, the simple procedure allows the assay of many samples at the same time.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0735-9640
e-ISSN
0735-9640
Journal
Plant Molecular Biology Reporter
Quellenangaben
Volume: 14,
Issue: 2,
Pages: 143-148
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)