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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Ozone-induced metabolic changes in Scots pine (Pinus sylvestris L.): mRNA isolation and analysis of in vitro translated proteins.
Can. J. For. Res. 24, 2030-2033 (1994)
A rapid isolation procedure for intact poly(A)+ RNA from Scots pine (Pinussylvestris L.) is described. Translation products of poly(A)+ RNA isolated from 6-week-old Scots pine seedlings treated with ozone (0.3 μL/L) for 8 h were analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Both qualitative and quantitative differences in the translation products were observed after ozone fumigation. Expression of specific mRNAs, coding for stress-induced polypeptides between 12 kD and 50 kD, was demonstrated after 8 h of ozone treatment.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0045-5067
Quellenangaben
Volume: 24,
Pages: 2030-2033
Publisher
NRC Research Press
Publishing Place
Ottawa, Ont.
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)