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Open Access Green as soon as Postprint is submitted to ZB.
Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay.
Planta 159, 216-221 (1983)
Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0032-0935
e-ISSN
1432-2048
Journal
Planta
Quellenangaben
Volume: 159,
Issue: 3,
Pages: 216-221
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)