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Fiamoncini, J.* ; Lima, T.M.* ; Hirabara, S.M.* ; Ecker, J. ; Gorjao, R.* ; Romanatto, T.* ; ELolimy, A.* ; Worsch, S. ; Laumen, H. ; Bader, B. ; Daniel, H.* ; Curi, R.*

Medium-chain dicarboxylic acylcarnitines as markers of n-3 PUFA-induced peroxisomal oxidation of fatty acids.

Mol. Nutr. Food Res. 59, 1573-1583 (2015)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
ScopeOmega-3 polyunsaturated fatty acids (n-3 PUFA) found in fish oil activate PPAR-, stimulate peroxisomal fatty acid (FA) -oxidation and prevent impairments on glucose homeostasis. Methods and resultsGlucose metabolism and FA oxidation were studied in C57/Bl6 mice fed with diets containing either 3.6 and 31.5% fish oil or lard. To assess the effects of peroxisomal proliferation on FA oxidation independent of n-3 PUFA intake, mice were treated with the PPAR- agonist WY-14643. n-3 PUFA-fed mice were protected from glucose intolerance and dyslipidemia compared to animals fed a lard-based high-fat diet. Most importantly, mice fed on the hyperlipidic diet based on fish oil as well as the WY-14643 treated mice showed twofold increase of odd, medium-chain, dicarboxylic acylcarnitines in the liver suggesting that not only -oxidation, but also - and -oxidation of FA were increased. Finally, an oxidation assay using liver homogenates and palmitic acid as substrate revealed an over tenfold increased production of similar acylcarnitines, indicating that FA are their precursors. ConclusionThis study shows at the metabolite level that peroxisome proliferation induced either by fish oil or WY-14643 is associated with increased - and -oxidation of FA producing specific acylcarnitines that can be utilized as biomarkers of peroxisomal FA oxidation.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Alpha-oxidation ; Beta-oxidation ; Peroxisomes ; Omega-3 Fatty Acids ; Omega-oxidation; Rat-liver Peroxisomes; Insulin-resistance; Ppar-alpha; Fish-oil; Beta-oxidation; Messenger-rna; Plasma-lipids; Acyl-coa; Expression; Accumulation
ISSN (print) / ISBN 1613-4125
e-ISSN 1613-4133
Journal Molecular Nutrition and Food Research
Quellenangaben Volume: 59, Issue: 8, Pages: 1573-1583 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Nutrigenomics and Type 2 Diabetes (KKG-KDN)