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Schwarz, M. ; Aguirre Bueno, J. ; Bühler, A. ; Omar, M. ; Ntziachristos, V.

Visualization of the microcirculatory network in skin by high frequency optoacoustic mesoscopy.

Proc. SPIE 9539:95390J (2015)
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Optoacoustic (photoacoustic) imaging has a high potential for imaging melanin-rich structures in skin and the microvasculature of the dermis due to the natural chromophores (de)oxyhemoglobin, and melanin. The vascular network in human dermis comprises a large network of arterioles, capillaries, and venules, ranging from 5 μm to more than 100 μm in diameter. The frequency spectrum of the microcirculatory network in human skin is intrinsically broadband, due to the large variety in size of absorbers. In our group we have developed raster-scan optoacoustic mesoscopy (RSOM) that applies a 100 MHz transducer with ultra-wide bandwidth in raster-scan mode achieving lateral resolution of 18 μm. In this study, we applied high frequency RSOM to imaging human skin in a healthy volunteer. We analyzed the frequency spectrum of anatomical structures with respect to depth and show that frequencies >60 MHz contain valuable information of structures in the epidermis and the microvasculature of the papillary dermis. We illustrate that RSOM is capable of visualizing the fine vascular network at and beneath the epidermal-dermal junction, revealing the vascular fingerprint of glabrous skin, as well as the larger venules deeper inside the dermis. We evaluate the ability of the RSOM system in measuring epidermal thickness in both hairy and glabrous skin. Finally, we showcase the capability of RSOM in visualizing benign nevi that will potentially help in imaging the penetration depth of melanoma.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Frequency Analysis ; Mesoscopy ; Microcirculation ; Optoacoustic ; Photoacoustic ; Skin
ISSN (print) / ISBN 0277-786X
e-ISSN 1996-756X
Conference Title Opto-Acoustic Methods and Applications in Biophotonics II
Conference Date 22-23 June 2015
Conference Location Munich, Germany
Journal Proceedings of SPIE
Quellenangaben Volume: 9539, Issue: , Pages: , Article Number: 95390J Supplement: ,
Publisher SPIE
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)