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Soliman, D. ; Tserevelakis, G.J.* ; Omar, M. ; Ntziachristos, V.

Hybrid label-free Multiphoton and Optoacoustic Microscopy (MPOM).

Proc. SPIE 9539:953908 (2015)
Publ. Version/Full Text DOI
Open Access Green as soon as Postprint is submitted to ZB.
Many biological applications require a simultaneous observation of different anatomical features. However, unless potentially harmful staining of the specimens is employed, individual microscopy techniques do generally not provide multi-contrast capabilities. We present a hybrid microscope integrating optoacoustic microscopy and multiphoton microscopy, including second-harmonic generation, into a single device. This combined multiphoton and optoacoustic microscope (MPOM) offers visualization of a broad range of structures by employing different contrast mechanisms and at the same time enables pure label-free imaging of biological systems. We investigate the relative performance of the two microscopy modalities and demonstrate their multi-contrast abilities through the label-free imaging of a zebrafish larva ex vivo, simultaneously visualizing muscles and pigments. This hybrid microscopy application bears great potential for developmental biology studies, enabling more comprehensive information to be obtained from biological specimens without the necessity of staining.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Label-free Imaging ; Microscopy ; Multimodal Imaging ; Nonlinear Microscopy ; Photoacoustic Imaging
ISSN (print) / ISBN 0277-786X
e-ISSN 1996-756X
Conference Title Opto-Acoustic Methods and Applications in Biophotonics II
Conference Date 22-23 June 2015
Conference Location Munich, Germany
Journal Proceedings of SPIE
Quellenangaben Volume: 9539, Issue: , Pages: , Article Number: 953908 Supplement: ,
Publisher SPIE
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)