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Open Access Green as soon as Postprint is submitted to ZB.
Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging.
Laser Photon. Rev. 9, L29-L34 (2015)
Intravital imaging of large specimens is intrinsically challenging for postembryonic studies. Selective plane illumination microscopy (SPIM) has been introduced to volumetrically visualize organisms used in developmental biology and experimental genetics. Ideally suited for imaging transparent samples, SPIM can offer high frame rate imaging with optical microscopy resolutions and low phototoxicity. However, its performance quickly deteriorates when applied to opaque tissues. To overcome this limitation, SPIM optics were merged with optical and optoacoustic (photoacoustic) readouts. The performance of this hybrid imaging system was characterized using various phantoms and by imaging a highly scattering ex vivo juvenile zebrafish. The results revealed the system's enhanced capability over that of conventional SPIM for high-resolution imaging over extended depths of scattering content. The approach described here may enable future visualization of organisms throughout their entire development, encompassing regimes in which the tissue may become opaque.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Biological Imaging ; Hybrid Imaging Systems ; Light-sheet Microscopy ; Optoacoustic Imaging
ISSN (print) / ISBN
1863-8880
e-ISSN
1863-8899
Journal
Laser & Photonics Reviews
Quellenangaben
Volume: 9,
Issue: 5,
Pages: L29-L34
Publisher
Wiley
Publishing Place
Weinheim
Non-patent literature
Publications
Reviewing status
Peer reviewed