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Filipczyk, A. ; Marr, C. ; Hastreiter, S. ; Feigelman, J. ; Schwarzfischer, M. ; Hoppe, P.S. ; Loeffler, D. ; Kokkaliaris, K.D. ; Endele, M. ; Schauberger, B. ; Hilsenbeck, O. ; Skylaki, S. ; Hasenauer, J. ; Anastassiadis, K.* ; Theis, F.J. ; Schroeder, T.

Network plasticity of pluripotency transcription factors in embryonic stem cells.

Nat. Cell Biol. 17, 1235-1246 (2015)
Supplement DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Transcription factor (TF) networks are thought to regulate embryonic stem cell (ESC) pluripotency. However, TF expression dynamics and regulatory mechanisms are poorly understood. We use reporter mouse ESC lines allowing non-invasive quantification of Nanog or Oct4 protein levels and continuous long-term single-cell tracking and quantification over many generations to reveal diverse TF protein expression dynamics. For cells with low Nanog expression, we identified two distinct colony types: one re-expressed Nanog in a mosaic pattern, and the other did not re-express Nanog over many generations. Although both expressed pluripotency markers, they exhibited differences in their TF protein correlation networks and differentiation propensities. Sister cell analysis revealed that differences in Nanog levels are not necessarily accompanied by differences in the expression of other pluripotency factors. Thus, regulatory interactions of pluripotency TFs are less stringently implemented in individual self-renewing ESCs than assumed at present.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2015
HGF-reported in Year 2015
ISSN (print) / ISBN 1465-7392
e-ISSN 1476-4679
Journal Nature Cell Biology
Quellenangaben Volume: 17, Issue: 10, Pages: 1235-1246 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)
Institute of Computational Biology (ICB)
POF-Topic(s) 30204 - Cell Programming and Repair
30205 - Bioengineering and Digital Health
Research field(s) Stem Cell and Neuroscience
Enabling and Novel Technologies
PSP Element(s) G-500800-001
G-503800-001
G-553800-001
PubMed ID 26389663
DOI 10.1038/ncb3237
WOS ID WOS:000362213500004
Scopus ID 84942981648
Scopus ID 84941905451
Erfassungsdatum 2015-09-30
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