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Open Access Green as soon as Postprint is submitted to ZB.
RNA fingerprinting of microbial community in the rhizosphere soil of grain legumes.
FEMS Microbiol. Lett. 240, 181-186 (2004)
Microbial structural and expression profiles of the rhizospheres of three legumes, faba beans, peas and white lupin, were compared by RNA-arbitrarily primed PCR technique. Two different primers, M 13 reverse and 10-mer primers, were used in the amplification and products resolved on non-denaturing polyacrylamide gel. With both DNA and RNA profiles Lupinus and Pisum rhizospheres were more similar to each other than to Vicia rhizosphere. The RAP-PCR products were also dot blotted and probed for bacterial peptidase transcripts. Plant-dependent rhizosphere effect was evident by the marked absence of transcripts for bacterial neutral metallopeptidase in Lupinus rhizosphere. The results of dot blot were further confirmed by RT-PCR for the expression of bacterial neutral metallopeptidase in the three rhizospheres
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
RNA extraction; RAP-PCR; rhizosphere; bacterial proteases; expression profiles
ISSN (print) / ISBN
0378-1097
e-ISSN
1574-6968
Journal
FEMS Microbiology Letters
Quellenangaben
Volume: 240,
Pages: 181-186
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Soil Ecology (IBOE)