PuSH - Publication Server of Helmholtz Zentrum München

Dean-Ben, X.L. ; Stiel, A.C.* ; Jiang, Y. ; Ntziachristos, V. ; Westmeyer, G.G. ; Razansky, D.

Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography.

Opt. Lett. 40, 4691-4694 (2015)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Discerning the accurate distribution of chromophores and biomarkers by means of optoacoustic imaging is commonly challenged by the highly heterogeneous excitation light patterns resulting from strong spatial variations of tissue scattering and absorption. Here we used the light-fluence dependent switching kinetics of reversibly switchable fluorescent proteins (RSFPs), in combination with real-time acquisition of volumetric multi-spectral optoacoustic data to correct for the light fluence distribution deep in scattering media. The new approach allows for dynamic fluence correction in time-resolved imaging, e.g., of moving organs, and can be extended to work with a large palette of available synthetic and genetically encoded photochromic substances for multiplexed wavelength-specific fluence normalization.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0146-9592
e-ISSN 1539-4794
Journal Optics Letters
Quellenangaben Volume: 40, Issue: 20, Pages: 4691-4694 Article Number: , Supplement: ,
Publisher Optical Society of America (OSA)
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)