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Open Access Green as soon as Postprint is submitted to ZB.
Genome editing in mice using TALE nucleases.
Methods Mol. Biol. 1338, 229-243 (2016)
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Gene Targeting ; Knock-in ; Knockout ; Mouse ; One-cell Embryo ; Talen
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
ISBN
978-1-4939-2931-3
Conference Title
TALENs
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1338,
Pages: 229-243
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)