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Müller, G.

Assays for insulin and insulin-like metabolic activity based on hepatocytes, myocytes and diaphragms.

In: Drug Discovery and Evaluation: Pharmacological Assays. Cham, Switzerland: Springer, 2016. 2723-2780
DOI
Despite the eminent importance of studies with primary and cultured adipocytes or adipose tissues on the basis of their physiological role in the regulation of lipid and carbohydrate metabolism in humans in combination with the relative low expenditure in preparing adipocytes of high quality and number, compounds and drug candidates for future antidiabetic and antiobesity therapy have to be analyzed for their effects in primary and cultured hepatocytes and myocytes or liver and muscle tissues, too. In principle, the majority of the assays described above for adipocytes can be adapted for the use with hepatocytes and myocytes. However, the following selection takes into account the relative contribution of each process monitored to its role in the whole-body regulation of intermediary metabolism in the normal and disease state. Moreover, technical aspects, such as requirement for a special equipment and applicability in throughput screening assays for drug discovery, were additional criteria.
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Publication type Article: Edited volume or book chapter
Editors Hock, F.J.*
Corresponding Author
e-ISSN 978-3-319-05392-9
ISBN 978-3-319-05391-2
Book Volume Title Drug Discovery and Evaluation: Pharmacological Assays
Quellenangaben Volume: , Issue: , Pages: 2723-2780 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Cham, Switzerland
Non-patent literature Publications
Institute(s) Institute of Diabetes and Obesity (IDO)