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Müller, G.

Assays for insulin and insulin-like regulation of gene and protein expression.

In: Drug Discovery and Evaluation: Pharmacological Assays. Cham, Switzerland: Springer, 2016. 2895-2934
DOI
Part of the blood glucose-lowering activity of insulin is based on massive and specific up- or downregulation of the gene expression for proteins/enzymes which directly regulate carbohydrate and lipid metabolism (e.g., GLUT4, PEPCK). Furthermore, changes in the intermediary metabolism provoked by insulin may secondarily lead to alterations in the gene/protein expression of other signaling/metabolic pathways. Consequently, it is important for the identification as well as characterization of compounds/drug candidates with insulin-like activity to analyze their effect on the expression of gene and proteins. For practical reasons, this analysis can be restricted to those genes/proteins which according to the present knowledge are relevant for mode of action of the compound/drug candidate. However, undoubtedly whole genome or proteome searches for changes in the expression levels have important advantages, in particular due to its unbiased nature. However, they require considerable experimental expenditure and time on basis of conventional technology. This may change completely with the successful introduction of protein chips and DNA microarrays for the study of protein and gene expression and further increase its attractiveness for routine application in characterization of novel compounds/drug candidates.
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Publication type Article: Edited volume or book chapter
Editors Hock, F.J.*
Corresponding Author
e-ISSN 978-3-319-05392-9
ISBN 978-3-319-05391-2
Book Volume Title Drug Discovery and Evaluation: Pharmacological Assays
Quellenangaben Volume: , Issue: , Pages: 2895-2934 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Cham, Switzerland
Non-patent literature Publications
Institute(s) Institute of Diabetes and Obesity (IDO)