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Jung, B. ; Padula, D. ; Burtscher, I. ; Landerer, C. ; Lutter, D. ; Theis, F.J. ; Messias, A.C. ; Geerlof, A. ; Sattler, M. ; Kremmer, E. ; Boldt, K. ; Ueffing, M. ; Lickert, H.

Pitchfork and Gprasp2 target smoothened to the primary cilium for Hedgehog pathway activation.

PLoS ONE 11:e0149477 (2016)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The seven-transmembrane receptor Smoothened (Smo) activates all Hedgehog (Hh) signaling by translocation into the primary cilia (PC), but how this is regulated is not well understood. Here we show that Pitchfork (Pifo) and the G protein-coupled receptor associated sorting protein 2 (Gprasp2) are essential components of an Hh induced ciliary targeting complex able to regulate Smo translocation to the PC. Depletion of Pifo or Gprasp2 leads to failure of Smo translocation to the PC and lack of Hh target gene activation. Together, our results identify a novel protein complex that is regulated by Hh signaling and required for Smo ciliary trafficking and Hh pathway activation.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Protein-coupled Receptors; Intraflagellar Transport Proteins; Embryonic Stem-cells; Neuronal Cilia; Localization; Mice; Identification; Ciliopathies; Ciliogenesis; Trafficking
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 11, Issue: 2, Pages: , Article Number: e0149477 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Regeneration Research (IDR)
Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Institute of Structural Biology (STB)
Institute of Molecular Immunology (IMI)
CF Metabolomics & Proteomics (CF-MPC)