PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Jung, B. ; Padula, D. ; Burtscher, I. ; Landerer, C. ; Lutter, D. ; Theis, F.J. ; Messias, A.C. ; Geerlof, A. ; Sattler, M. ; Kremmer, E. ; Boldt, K. ; Ueffing, M. ; Lickert, H.

Pitchfork and Gprasp2 target smoothened to the primary cilium for Hedgehog pathway activation.

PLoS ONE 11:e0149477 (2016)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The seven-transmembrane receptor Smoothened (Smo) activates all Hedgehog (Hh) signaling by translocation into the primary cilia (PC), but how this is regulated is not well understood. Here we show that Pitchfork (Pifo) and the G protein-coupled receptor associated sorting protein 2 (Gprasp2) are essential components of an Hh induced ciliary targeting complex able to regulate Smo translocation to the PC. Depletion of Pifo or Gprasp2 leads to failure of Smo translocation to the PC and lack of Hh target gene activation. Together, our results identify a novel protein complex that is regulated by Hh signaling and required for Smo ciliary trafficking and Hh pathway activation.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
3.057
1.034
20
21
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Protein-coupled Receptors; Intraflagellar Transport Proteins; Embryonic Stem-cells; Neuronal Cilia; Localization; Mice; Identification; Ciliopathies; Ciliogenesis; Trafficking
Language english
Publication Year 2016
HGF-reported in Year 2016
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 11, Issue: 2, Pages: , Article Number: e0149477 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Regeneration Research (IDR)
Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Institute of Structural Biology (STB)
Institute of Molecular Immunology (IMI)
CF Metabolomics & Proteomics (CF-MPC)
POF-Topic(s) 30201 - Metabolic Health
30205 - Bioengineering and Digital Health
30203 - Molecular Targets and Therapies
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Helmholtz Diabetes Center
Enabling and Novel Technologies
Immune Response and Infection
PSP Element(s) G-502300-001
G-502200-001
G-503800-001
G-503000-001
G-503000-003
G-501793-001
G-505700-001
PubMed ID 26901434
DOI 10.1371/journal.pone.0149477
WOS ID WOS:000371276100080
Scopus ID 84960538904
Erfassungsdatum 2016-02-25
[➜Report correction]