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Elemental bioimaging and speciation analysis for the investigation of Wilson's disease using μXRF and XANES.
Metallomics 8, 648-653 (2016)
A liver biopsy specimen from a Wilson's disease (WD) patient was analyzed by means of micro-X-ray fluorescence (μXRF) spectroscopy to determine the elemental distribution. First, bench-top μXRF was utilized for a coarse scan of the sample under laboratory conditions. The resulting distribution maps of copper and iron enabled the determination of a region of interest (ROI) for further analysis. In order to obtain more detailed elemental information, this ROI was analyzed by synchrotron radiation (SR)-based μXRF with a beam size of 4 μm offering a resolution at the cellular level. Distribution maps of additional elements to copper and iron like zinc and manganese were obtained due to a higher sensitivity of SR-μXRF. In addition to this, X-ray absorption near edge structure spectroscopy (XANES) was performed to identify the oxidation states of copper in WD. This speciation analysis indicated a mixture of copper(i) and copper(ii) within the WD liver tissue.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Ray-fluorescence Microscopy; Synchrotron-radiation; Oxidation-state; Copper; Gene; Spectroscopy; Spectrometry; Diagnosis; Atpase; Liver
ISSN (print) / ISBN
1756-5901
e-ISSN
1756-591X
Journal
Metallomics
Quellenangaben
Volume: 8,
Issue: 7,
Pages: 648-653
Publisher
Royal Society of Chemistry (RSC)
Publishing Place
Cambridge
Non-patent literature
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Reviewing status
Peer reviewed