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Travers, A.* ; Schneider, R.* ; Muskhelishvili, G.*

DNA supercoiling and transcription in Escherichia coli: The FIS connection.

Biochimie 83, 213-217 (2001)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The nucleoid-associated protein FIS modulates the topology of DNA in a growth-phase dependent manner functioning homeostatically to counteract excessive levels of negative superhelicity. We propose that this is achieved by at least two mechanisms: the physical constraint of low levels of negative superhelicity by FIS binding to DNA and by a reduction in the expression and effectiveness of DNA gyrase. In addition, high levels of expression of the fis gene do themselves require a high negative superhelical density. On DNA substrates containing phased high affinity binding sites, as exemplified by the upstream activating sequence of the tyrT promoter, FIS forms tightly bent DNA structures, or microloops, that are necessary for the optimal expression of the promoter. We suggest that these microloops compensate in part for the FIS-induced lowering of the superhelical density.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2001
HGF-reported in Year 0
ISSN (print) / ISBN 0300-9084
e-ISSN 1638-6183
Journal Biochimie
Quellenangaben Volume: 83, Issue: 2, Pages: 213-217 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Functional Epigenetics (IFE)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Helmholtz Diabetes Center
PSP Element(s) G-502800-001
PubMed ID 11278071
DOI 10.1016/S0300-9084(00)01217-7
Erfassungsdatum 2001-12-31
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