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Schneider, R.* ; Lurz, R.* ; Lüder, G.* ; Tolksdorf, C.* ; Travers, A.* ; Muskhelishvili, G.*

An architectural role of the Escherichia coli chromatin protein FIS in organising DNA.

Nucleic Acids Res. 29, 5107-5114 (2001)
DOI PMC
Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
The Escherichia coli chromatin protein FIS modulates the topology of DNA in a growth phase-dependent manner. In this study we have investigated the global effect of FIS binding on DNA architecture in vitro. We show that in supercoiled DNA molecules FIS binds at multiple sites in a non-random fashion and increases DNA branching. This global DNA reshaping effect is independent of the helical phasing of FIS binding sites. We propose, in addition to the previously inferred stabilisation of tightly bent DNA microloops in the upstream regions of certain promoters, that FIS may perform the distinct architectural function of organising branched plectonemes in the E.coli nucleoid.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Quellenangaben Volume: 29, Issue: 24, Pages: 5107-5114 Article Number: , Supplement: ,
Publisher Oxford University Press
Non-patent literature Publications
Reviewing status Peer reviewed