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Open Access Green as soon as Postprint is submitted to ZB.
A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli.
Mol. Microbiol. 34, 953-964 (1999)
In Escherichia coli, the transcriptional activity of many promoters is strongly dependent on the negative superhelical density of chromosomal DNA. This, in turn, varies with the growth phase, and is correlated with the overall activity of DNA gyrase, the major topoisomerase involved in the elevation of negative superhelicity. The DNA architectural protein FIS is a regulator of the metabolic reorganization of the cell during early exponential growth phase. We have previously shown that FIS modulates the superhelical density of plasmid DNA in vivo, and on binding reshapes the supercoiled DNA in vitro. Here, we show that, in addition, FIS represses the gyrA and gyrB promoters and reduces DNA gyrase activity. Our results indicate that FIS determines DNA topology both by regulation of topoisomerase activity and, as previously inferred, by directly reshaping DNA. We propose that FIS is involved in coupling cellular physiology to the topology of the bacterial chromosome.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0950-382x
e-ISSN
1365-2958
Journal
Molecular Microbiology
Quellenangaben
Volume: 34,
Issue: 5,
Pages: 953-964
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Functional Epigenetics (IFE)