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Ziegler-Birling, C.* ; Daujat, S.* ; Schneider, R.* ; Torres-Padilla, M.E.*

Dynamics of histone H3 acetylation in the nucleosome core during mouse pre-implantation development.

Epigenetics, DOI: 10.1080/15592294.2015.1103424 (2015)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
In mammals, the time period that follows fertilization is characterized by extensive chromatin remodeling, which enables epigenetic reprogramming of the gametes. Major changes in chromatin structure persist until the time of implantation, when the embryo develops into a blastocyst, which comprises the inner cell mass and the trophectoderm. Changes in DNA methylation, histone variant incorporation, and covalent modifications of the histones tails have been intensively studied during pre-implantation development. However, modifications within the core of the nucleosomes have not been systematically analyzed. Here, we report the first characterization and temporal analysis of three key acetylated residues in the core of the histone H3: H3K64ac, H3K122ac, and H3K56ac, all located at structurally important positions close to the DNA. We found that all three acetylations occur during pre-implantation development, but with different temporal kinetics. Globally, H3K64ac and H3K56ac were detected throughout cleavage stages, while H3K122ac was only weakly detectable during this time. Our work contributes to the understanding of the contribution of histone modifications in the core of the nucleosome to the "marking" of the newly established embryonic chromatin and unveils new modification pathways potentially involved in epigenetic reprogramming.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Mammalian Embryo ; Epigenetics ; Histone Acetylation ; Lateral Surface ; Nucleosome Core
ISSN (print) / ISBN 1559-2294
e-ISSN 1559-2308
Journal Epigenetics
Publisher Landes Bioscience
Publishing Place Austin, Tex.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)
Institute of Functional Epigenetics (IFE)