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Higher chromatin mobility supports totipotency and precedes pluripotency in vivo.
Genes Dev. 28, 1042-1047 (2014)
The fusion of the gametes upon fertilization results in the formation of a totipotent cell. Embryonic chromatin is expected to be able to support a large degree of plasticity. However, whether this plasticity relies on a particular conformation of the embryonic chromatin is unknown. Moreover, whether chromatin plasticity is functionally linked to cellular potency has not been addressed. Here, we adapted fluorescence recovery after photobleaching (FRAP) in the developing mouse embryo and show that mobility of the core histones H2A, H3.1, and H3.2 is unusually high in two-cell stage embryos and decreases as development proceeds. The transition toward pluripotency is accompanied by a decrease in histone mobility, and, upon lineage allocation, pluripotent cells retain higher mobility than the differentiated trophectoderm. Importantly, totipotent two-cell-like embryonic stem cells also display high core histone mobility, implying that reprogramming toward totipotency entails changes in chromatin mobility. Our data suggest that changes in chromatin dynamics underlie the transitions in cellular plasticity and that higher chromatin mobility is at the nuclear foundations of totipotency.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Fate ; Chromatin Dynamics ; Pluripotency ; Reprogramming ; Totipotent Cells
Language
english
Publication Year
2014
HGF-reported in Year
0
ISSN (print) / ISBN
0890-9369
e-ISSN
1549-5477
Journal
Genes and Development
Quellenangaben
Volume: 28,
Issue: 10,
Pages: 1042-1047
Publisher
Cold Spring Harbor Laboratory Press
Reviewing status
Peer reviewed
Institute(s)
Institute of Epigenetics and Stem Cells (IES)
POF-Topic(s)
30204 - Cell Programming and Repair
Research field(s)
Stem Cell and Neuroscience
PSP Element(s)
G-506200-001
PubMed ID
24831699
Erfassungsdatum
2014-12-31