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Oda, H.* ; Okamoto, I.* ; Murphy, N.* ; Chu, J.* ; Price, S.M.* ; Shen, M.M.* ; Torres-Padilla, M.E.* ; Heard, E.* ; Reinberg, D.*

Monomethylation of histone H4-lysine 20 is involved in chromosome structure and stability and is essential for mouse development.

Mol. Cell. Biol. 29, 2278-2295 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
PR-Set7/Set8/KMT5A is the sole enzyme known to catalyze monomethylation of histone H4 lysine 20 (H4K20) and is present only in multicellular organisms that compact a large fraction of their DNA. We found that mouse embryos that are homozygous null mutants for the gene PR-Set7 display early embryonic lethality prior to the eight-cell stage. Death was due to the absence of PR-Set7 catalytic activity, since microinjection of the wild type, but not a catalytically inactive version, into two-cell embryos rescued the phenotype. A lack of PR-Set7 activity resulted not only in depletion of H4K20me1 but also in reduced levels of the H4K20me2/3 marks catalyzed by the Suv4-20h1/h2 enzymes, implying that H4K20me1 may be essential for the function of these enzymes to ensure the dimethylated and trimethylated states. Embryonic stem cells that were inducibly deleted for PR-Set7 passed through an initial G(2)/M phase, but the progeny were defective at the subsequent S and G(2)/M phases, exhibiting a delay in their cell cycle, accumulation at G(2)/M, massive DNA damage, and improper mitotic chromosome condensation. Cell cycle analysis after synchronization indicated that the defects were a consequence of decreased H4K20me1 due to the absence of PR-Set7. Most importantly, the lack of H4K20me1 also resulted in defects in chromosome condensation in interphase nuclei. These results demonstrate the critical role of H4K20 monomethylation in mammals in a developmental context.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0270-7306
e-ISSN 1098-5549
Journal Molecular and Cellular Biology
Quellenangaben Volume: 29, Issue: 8, Pages: 2278-2295 Article Number: , Supplement: ,
Publisher American Society for Microbiology (ASM)
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)