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Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics".
Science 352:527 (2016)
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Li et al (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods' reliability to visualize specimen features at sub-100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.
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Publication type
Article: Journal article
Document type
Other: News Item
Keywords
Fluorescence Microscopy; Optical Resolution; Nanoscopy; Protein; Light; Reveals; Limit; Gfp
Language
english
Publication Year
2016
HGF-reported in Year
2016
ISSN (print) / ISBN
0036-8075
e-ISSN
1095-9203
Journal
Science
Quellenangaben
Volume: 352,
Issue: 6285,
Article Number: 527
Publisher
American Association for the Advancement of Science (AAAS)
Publishing Place
Washington
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s)
30205 - Bioengineering and Digital Health
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
G-505500-001
WOS ID
WOS:000374998600028
PubMed ID
27126030
Erfassungsdatum
2016-05-01