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Open Access Green as soon as Postprint is submitted to ZB.
qPCR zur quantitativen Validierung von Metagenomdaten.
BioSpektrum 22, 265-269 (2016)
Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0947-0867
e-ISSN
1868-6249
Journal
BioSpektrum
Quellenangaben
Volume: 22,
Issue: 3,
Pages: 265-269
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Research Unit Comparative Microbiome Analysis (COMI)