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Open Access Green as soon as Postprint is submitted to ZB.
Serine protease inhibitor lymphoepithelial Kazal type-related inhibitor tends to be decreased in atopic dermatitis.
J. Eur. Acad. Dermatol. Venereol. 23, 1263-1266 (2009)
BACKGROUND: A pathogenic role of serine protease inhibitor lymphoepithelial Kazal type-related inhibitor (LEKTI) in atopic dermatitis (AD) is currently in intense debate. Analyses of an association between genetic polymorphisms of SPINK5 and atopic diseases revealed contradictory results. Herein, we assessed the role of LEKTI in AD at an expressional and functional level. METHODS: The expression of LEKTI and its inhibitory capacity was measured by real-time polymerase chain reaction and hydrolytic activity assay, respectively, in keratinocyte cell cultures of three AD patients in comparison to cultures of healthy individuals (5x) and Netherton (NS) patients (3x). RESULTS: Expression of LEKTI was significantly decreased in AD vs. healthy volunteers. Due to reduced protease inhibition, trypsin-like hydrolytic activity in AD was slightly increased, although not significantly. CONCLUSIONS: Even though the number of investigated subjects was small and hydrolytic activity was only slightly increased, the results denote that LEKTI might be diminished in AD. The study also disclosed the necessity of functional analyses in addition to genetic investigations to gain further and more detailed insights into the role of LEKTI in AD.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0926-9959
e-ISSN
1468-3083
Quellenangaben
Volume: 23,
Issue: 11,
Pages: 1263-1266
Publisher
Wiley
Reviewing status
Peer reviewed
Institute(s)
Institute for Allergy Research (IAF)