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Pallua, J.D.* ; Schaefer, G.* ; Bittner, L.K.* ; Pezzei, C.* ; Huck-Pezzei, V.* ; Schoenbichler, S.A.* ; Meding, S. ; Rauser, S. ; Walch, A.K. ; Handler, M.* ; Netzer, M.* ; Osl, M.* ; Seger, M.* ; Pfeifer, B.* ; Baumgartner, C.* ; Lindner, H.* ; Kremser, L.* ; Sarg, B.* ; Klocker, H.* ; Bartsch, G.* ; Bonn, G.K.* ; Huck, C.W.*

Matrix-assisted laser desorption-ionization imaging mass spectrometry for direct tissue analysis.

LC GC N. Am. Suppl. S, Suppl., 21-28 (2011)

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Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool in histopathological characterization and represents a modern analytical technique, enabling two-dimensional detection of molecular components of biological samples. Using this method, it is possible to investigate the spatial distribution of proteins, lipids, carbohydrates, cholesterols, nucleic acids, phospholipids, and small molecules in biological systems by in-situ analysis of cell cultures and tissue sections. Recently, MALDI-IMS has become an essential tool for tissue analyses in life science applications, offering global analysis of tissue samples. An advantage of this imaging technique is the acquisition of local molecular expression profiles up to the microscopic level, while maintaining the topographic integrity of the tissue by avoiding time-consuming extraction, purification, or separation steps, respectively. With MALDI-IMS it is possible to determine the distribution of hundreds of unknown compounds in a single measurement, allowing rapid probing of the tissues' biochemistry. Moreover, MALDI-IMS results include qualitative and semiquantitative information, providing unique chemi-morphological information about the tissue status, which represents an important benefit for future analytical interpretation of pathological changes of a tissue. This article summarizes the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords sample preparation; maldi-ms; capture microdissection; small molecules; drug discovery; breast-cancer; ion-trap; proteomics; proteins; desorption/ionization

ISSN (print) / ISBN 1527-5949

e-ISSN 1939-1889

Journal LC GC North America

Quellenangaben Volume: Suppl. S, Pages: 21-28, Supplement: Suppl.

Publisher Advanstar Communications

Publishing Place Duluth, MN, USA

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)