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Badie, C.* ; Blachowicz, A.* ; Barjaktarovic, Z. ; Finnon, R.* ; Michaux, A.* ; Sarioglu, H. ; Brown, N.* ; Manning, G.* ; Benotmane, M.A.* ; Tapio, S. ; Polanska, J.* ; Bouffler, S.D.*

Transcriptomic and proteomic analysis of mouse radiation-induced Acute Myeloid Leukaemia (AML).

Oncotarget 7, 40461-40480 (2016)
Publ. Version/Full Text Research data DOI PMC
Creative Commons Lizenzvertrag
A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50% of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher. An AML 'signature' of 17 genes/proteins commonly deregulated in primary AMLs and cell lines compared to HPSCs was identified and validated using human AML transcriptome data. This also distinguishes primary AMLs from cell lines and includes proteins such as Coronin 1, pontin/RUVBL1 and Myeloperoxidase commonly implicated in human AML. C-Myc was identified as having a key role in radiation leukaemogenesis. These data identify novel candidates relevant to mouse radiation AML pathogenesis, and confirm that pathways of leukaemogenesis in the mouse and human share substantial commonality.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Acute Myeloid Leukaemia ; Gene Expression ; Ionising Radiation ; Mouse ; Protein Expression; Chromosome 2 Deletions; Ionizing-radiation; C57bl/6 Mice; Stem-cells; Pu.1; Gene; Expression; Mutations; Protein; Leukemogenesis
ISSN (print) / ISBN 1949-2553
e-ISSN 1949-2553
Journal OncoTarget
Quellenangaben Volume: 7, Issue: 26, Pages: 40461-40480 Article Number: , Supplement: ,
Publisher Impact Journals LLC
Publishing Place Albany
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)