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Comparative study to the use of decellularized alpha-Gal KO pig lungs for xenogeneic lung transplantation.
Tissue Eng. Part C Meth. 22, 725-739 (2016)
Use of decellularized pig lung scaffolds recellularized with human lung cells in xenogeneic transplantation is potentially limited by expression of immunogenic galactosylated cell surface glycoproteins. Use of alpha 1,3 galactosyltransferase knock out (α-gal KO) pigs presents a potential alternative. Comparison of decellularized wild type (WT) vs α-gal KO pig lungs by histologic, immunohistochemical, and mass spectrometric techniques demonstrated no obvious differences in histologic structure was observed but an approximate 25% difference in retention of residual proteins including retention of α-galactosylated epitopes in WT lungs. Comparable robust initial recellularization and subsequent growth and proliferation was observed in wild type (WT) vs α-gal KO pig lungs following compartmental inoculation of human lung bronchial epithelial cells (HBE), human lung fibroblasts (HLF), and human bone marrow-derived mesenchymal stromal cells (MSCS) and of human pulmonary vascular endothelial cells (CBF). These initial sutides support further investigation of decellularized α-Gal KO pig lungs for xenogeneic lung regeneration.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Epithelial-cells; Immune-response; Stem-cells; Re-cellularization; High-throughput; Acellular Human; Clinical-scale; Porcine Lungs; Heart-valves; Scaffolds
ISSN (print) / ISBN
1937-3384
e-ISSN
1937-3392
Quellenangaben
Volume: 22,
Issue: 8,
Pages: 725-739
Publisher
Mary Ann Liebert
Publishing Place
New Rochelle
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Lung Health and Immunity (LHI)