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Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions.
Proc. SPIE 9708:97083B (2016)
Publ. Version/Full Text
DOI
We present a multi-scale imaging system that integrates five optoacoustic and multi-photon modalities into the same device. The hybrid microscope offers a unique zoom-in ability by allowing for optoacoustic microscopy and mesoscopy scans of the sample within the same imaging framework. Furthermore, by combining several label-free modalities, we are able to visualize a broad range of anatomical features, taking advantage of their complementary contrast mechanisms. We characterize the spatial resolution and relative orientation of the different sub-modalities and demonstrate the system's performance by the imaging of several model organisms ex vivo. The presented ability to dynamically vary scanning volume and resolution together with its multi-contrast and label-free imaging capabilities make the hybrid microscope a promising tool for comprehensive biological imaging.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Mesoscopy
ISSN (print) / ISBN
0277-786X
e-ISSN
1996-756X
Conference Title
Photons Plus Ultrasound: Imaging and Sensing 2016
Conference Date
14-17 February 2017
Conference Location
San Francisco, CA, USA
Journal
Proceedings of SPIE
Quellenangaben
Volume: 9708,
Article Number: 97083B
Publisher
SPIE
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)