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Schlosser, E.* ; Mueller, M.* ; Fischer, S.* ; Basta, S.* ; Busch, D.H. ; Gander, B.* ; Groettrup, M.*

TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses.

Vaccine 26, 1626-1637 (2008)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(D,L-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2K(b) tetramer positive CTLs, production of IFN-gamma, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Biodegradable microspheres; Viral infection; Cytotoxic T cells
ISSN (print) / ISBN 0264-410X
e-ISSN 1358-8745
Journal Vaccine
Quellenangaben Volume: 26, Issue: 13, Pages: 1626-1637 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Immune Monitoring (Platform) (IMI-KIM)
Institute of Virology (VIRO)