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Klanert, G.* ; Jadhav, V.* ; Shanmukam, V.* ; Diendorfer, A.* ; Karbiener, M.* ; Scheideler, M. ; Bort, J.H.* ; Grillari, J.* ; Hackl, M.* ; Borth, N.*

A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines.

J. Biotechnol. 235, 150-161 (2016)

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	Open Access Gold (Paid Option)

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As Chinese Hamster Ovary (CHO) cells are the cell line of choice for the production of human-like recombinant proteins, there is interest in genetic optimization of host cell lines to overcome certain limitations in their growth rate and protein secretion. At the same time, a detailed understanding of these processes could be used to advantage by identification of marker transcripts that characterize states of performance.In this context, microRNAs (miRNAs) that exhibit a robust correlation to the growth rate of CHO cells were determined by analyzing miRNA expression profiles in a comprehensive collection of 46 samples including CHO-K1, CHO-S and CHO-DUKXB11, which were adapted to various culture conditions, and analyzed in different growth stages using microarrays. By applying Spearman or Pearson correlation coefficient criteria of. >. |0.6|, miRNAs with high correlation to the overall growth, or growth rates observed in exponential, serum-free, and serum-free exponential phase were identified. An overlap of twelve miRNAs common for all sample sets was revealed, with nine positively and three negatively correlating miRNAs.The here identified panel of miRNAs can help to understand growth regulation in CHO cells and contains putative engineering targets as well as biomarkers for cell lines with advantageous growth characteristics.

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Publication type Article: Journal article

Document type Review

Corresponding Author

Keywords Cho; Hamster Ovary Cells; Recombinant Protein-production; Hepatocellular-carcinoma; Messenger-rna; Posttranscriptional Regulation; Colorectal-cancer; Mesenchymal Transition; Conserved Micrornas; Cerebral-ischemia; Tumor-suppressor

ISSN (print) / ISBN 0168-1656

e-ISSN 1873-4863

Journal Journal of Biotechnology

Quellenangaben Volume: 235, Pages: 150-161

Publisher Elsevier

Publishing Place Amsterdam

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Diabetes and Cancer (IDC)