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Rothballer, M. ; Eckert, B. ; Schmid, M. ; Fekete, A. ; Schloter, M. ; Lehner, A.* ; Pollmann, S.* ; Hartmann, A.

Endophytic root colonization of gramineous plants by Herbaspirillum frisingense.

FEMS Microbiol. Ecol. 66, 85-95 (2008)
DOI
Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Herbaspirillum frisingense is a diazotrophic betaproteobacterium isolated from C4-energy plants, for example Miscanthus sinensis. To demonstrate endophytic colonization unequivocally, immunological labeling techniques using monospecific polyclonal antibodies against two H. frisingense strains and green fluorescent protein (GFP)-fluorescence tagging were applied. The polyclonal antibodies enabled specific in situ identification and very detailed localization of H. frisingense isolates Mb11 and GSF30(T) within roots of Miscanthusxgiganteus seedlings. Three days after inoculation, cells were found inside root cortex cells and after 7 days they were colonizing the vascular tissue in the central cylinder. GFP-tagged H. frisingense strains could be detected and localized in uncut root material by confocal laser scanning microscopy and were found as endophytes in cortex cells, intercellular spaces and the central cylinder of barley roots. Concerning the production of potential plant effector molecules, H. frisingense strain GSF30(T) tested positive for the production of indole-3-acetic acid, while Mb11 was shown to produce N-acylhomoserine lactones, and both strains were able to utilize 1-aminocyclopropane-1-carboxylate (ACC), providing an indication of the activity of an ACC-deaminase. These results clearly present H. frisingense as a true plant endophyte and, although initial greenhouse experiments did not lead to clear plant growth stimulation, demonstrate the potential of this species for beneficial effects on the growth of crop plants.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Herbaspirillum frisingense; Miscanthus; barley; endophytic colonization; IAA production; AHL production
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Journal FEMS Microbiology Ecology
Quellenangaben Volume: 66, Issue: 1, Pages: 85-95 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Oxford
Reviewing status Peer reviewed
Institute(s) Research Unit Microbe-Plant Interactions (AMP)
Institute of Soil Ecology (IBOE)
Institute of Ecological Chemistry (IOEC)